Commercially available methods were used to determine whether the patients had diabetes, and restriction enzyme-based genotyping was used to determine the APOE genotypes. Forty-three Alzheimer´s patients were compared with 43 control subjects, paired by age and gender. Clinical studies were carried out by surveying the clinical histories in a group of patients in the province of Antioquia, Colombia. To determine if a correlation exists between APOE genotype, diabetes mellitus and Alzheimer´s disease. Among the risk factors, diabetes mellitus, and the ε4 isoform of the APOE gene have been amply demonstrated as increasing the risk of developing this disease. Numerous variables are associated with increased risk of developing this severe neurological disorder. Alzheimer´s disease is a multifactorial disease affecting approximately twenty million people worldwide. Oscar Alzate coordinated the project, helped with data analysis, and finalized the manuscript.Īll authors approved the final version of the manuscript. Jeong prepared the samples and performed the genotyping.Īll co-authors analyzed the results, and contributed in drafting the manuscript. Botero performed the initial preparation of the samples for laboratory analysis.Īna Corcimaru, Cristina Osorio and Joseph S. Rodríguez made clinical evaluations of all individuals in the study. Rodríguez and Gustavo A.Īlarcón prepared and designed the research project, made home visits to the patients and control individuals, applied epidemiological surveys, collected data and laboratory samples.
* These authors contributed equally to this work Jeong 2,4,5, and Oscar Alzate 1,2,4,5,6ġEscuela de Ciencias de la Salud, Universidad Pontificia Bolivariana, Medellín, ColombiaĢGrupo de Biología Estructural y Proteómica Group, Universidad Pontificia Bolivariana, Medellín, ColombiaģFundación Instituto Neurológico de Colombia (INDEC), Medellín, ColombiaĤSystems Proteomics Center, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, USAĥDepartment of Cell and Developmental Biology, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, USAĦProgram in Molecular Biology and Biotechnology, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, USA Alarcón 1, Ana Corcimaru 2,4,5, Cristina Osorio 2,4,6, Joseph S. Diabetes mellitus in patients withAlzheimer´s disease: clinical descriptionand correlation with the APOEgenotype in a sample population from the province of Antioquia, Colombia